Rattlesnake Creek is a tributary of Spring Brook in Lackawanna County, Pennsylvania, in the United States.

On the southern side of the lake, it flows south for several tenths of a mile, crossing Pennsylvania Route 690 and receiving the tributary Six Springs Creek from the left.

After a few miles, it turns southwest and reaches its confluence with Spring Brook in the Nesbitt Reservoir.

[1] Rattlesnake Creek joins Spring Brook 7.96 miles (12.81 km) upstream of its mouth.

However, some patches of Wisconsinan Ice-Contact Stratified Drift and alluvial fan are present, as are larger areas of a glacial or resedimented till known as Wisconsinan Till and bedrock consisting of conglomerate, sandstone, and shale.

[7] Below the second Pennsylvania Route 690 crossing, Rattlesnake Creek is entirely on private land and is closed to access.

[8] A concrete tee beam bridge carrying Pennsylvania Route 690 was constructed across Rattlesnake Creek in 1957.

[9] Wild trout naturally reproduce in Rattlesnake Creek from its headwaters downstream to its mouth.