In 1608, a red seal ship belonging to the Hinoe daimyō Arima Harunobu anchored in Macau after returning from Cambodia, where it had acquired a cargo of agarwood.

Alarmed by the situation, Captain-major André Pessoa responded with armed reinforcements, forcing the Japanese to take refuge in nearby houses.

However, Pessoa subsequently had the suspected ringleaders executed while allowing the others to leave Macau after they signed an affidavit absolving the Portuguese of any blame.

The rest were only allowed to board their ships back to Japan after signing a sworn statement accepting full responsibility for the incident and absolving the Portuguese of any blame.

[6] On May 10, 1609, the Portuguese carrack, known as either Nossa Senhora da Graça or Madre de Deus, departed Macau, carrying a rich cargo intended for the Japanese market.