Reverse northern blot

[1] Although DNA Microarrays and newer next-generation techniques have generally supplanted reverse northern blotting, it is still utilized today and provides a relatively cheap and easy means of defining expression of large sets of genes.

Expression levels can be quantified by increase or decrease in fluorescent or radioactive signal over a control treatment.

Therefore, reverse northern has been used as one means of confirming hits from DD-PCR, or sequences with altered expression levels.

[6] Arrays may be purchased from commercial suppliers tailored to research needs e.g. cancer, cell cycle, or toxicology microarrays, or may be generated for custom targets.

[7] Fluorescent or radioactive signals generated by hybridization of isolated sample cDNA probes will be proportional to the transcript's abundance in the tissue being studied.