Reverse transfection is a technique for the transfer of genetic material into cells.
Besides gelatin, atelocollagen and fibronectin are also successful transfection vectors for introducing foreign DNA into the cell nucleus.
It uses pressure control and a piezoelectric collar to squeeze out consistent drops of approximately 333 pL in volume.
After printing, the solution is allowed to dry up and the DNA-gelatin is stuck tightly in position on the array.
Second, 200ul of transfection mix is pipetted into one of the HybriWell ports; the mixture will distribute evenly over the array.
Third, the transfection mix is pipetted away and the HybriWell removed with a thin-tipped forceps.
In the first step of Effectene–DNA complex formation, the DNA is condensed by interaction with the enhancer in a defined-buffer system.