The state park adjoins the Rio Negro Right Bank Environmental Protection Area along its south east boundary, which is formed by the Puduari River.

[3] The region in which the Rio Negro State Park North Section lies has a humid tropical climate with average monthly temperatures no lower than 18 °C (64 °F).

[5] There are over 200 bird species including the rare pavonine cuckoo (Dromococcyx pavoninus), nocturnal curassow (Nothocrax urumutum), white-naped seedeater (Dolospingus fringilloides) and Pelzeln's tody-tyrant (Hemitriccus inornatus).

[5] Threats include extraction of lianas, gravel and sand, commercial fishing, military exercises, hunting, logging and unregulated tourism.

With the revised boundaries the north section was reduced to a smaller part of the right (west) bank of the Rio Negro, although it now extended further to west, and the south section was reduced to a smaller part of the left (east) bank of the river.

[7] Land ownership was complex, with areas belonging to the federal, state and municipal governments, to individuals and squatters.

[7] Part of the park is designated for use by the Ministry of Defence as a training site for the Navy Command Unit based in Manaus.

[9] Actions to implement the park began in 2004 when the Instituto de Proteção Ambiental do Amazonas (IPAAM) made the first meetings with the lower Rio Negro communities in the context of the Ecological Corridors Project, with the initial purpose of establishing a single council for the mosaic of state and federal conservation units in the lower Rio Negro.

[11] On 9 September 2014 a working group was established to review the limits of the Puduari-Solimões section of the Margem Direita do Rio Negro Environmental Protection Area and the Rio Negro Setor Norte State Park to compensate for land used to create the new campus for the Amazonas State University.