[4] After completing a PhD at the University of Vienna in 1994,[5] Mistrík continued his scientific career as a visiting scholar at the National Institute of Standards and Technology in Gaithersburg, Maryland.

In 1998, Mistrík founded the mass spectrometry, metabolomics and chemical analysis firm HighChem, which he still manages.

[6] Mistrík served as a member of the scientific steering committee in the METAcancer consortium, focused on the search for breast cancer biomarkers.

[7] He is the author of a patent for the identification of small molecules, including novel diagnostic markers of insidious diseases,[8] as well as Mass Frontier software.

[11] Mistrík leads the development of a cloud-based spectral tree database called mzCloud,[12] which enables the identification of multiple scientific problems.