The team launched alongside the rebranded men's Rochester Lancers NPSL side in April 2017.

Playing home games at the Aquinas Institute the team was winless in 2017.

For 2018 the team played at Marina Auto Stadium in downtown Rochester, NY.

The 2020 UWS season was cancelled due to the pandemic; returning to play in 2021 the team posted a record of 0–12.

[inconsistent] The Lady Lancers play home matches at Marina Auto Stadium