The Roman Catholic Diocese of Lucena (Latin: Dioecesis Lucenensis) is a diocese of the Roman Catholic Church in the Philippines with the seat in Lucena City.

The Roman Rite Latin Church diocese was erected in 1950 from the Archdiocese of Lipa to become a suffragan diocese of the archdiocese.

In 1977, the Diocese of Boac, which covers the parishes in the island-province of Marinduque, was created and separated from Lucena.

In 1984, the Diocese of Lucena was further divided with the creation of the Diocese of Gumaca, which covers the municipalities of Quezon province east of Gumaca, Quezon.

For the complete list of Roman Catholic churches in Quezon, see the Roman Catholic Diocese of Gumaca and Territorial Prelature of Infanta