The Roman Catholic Diocese of Trujillo (Latin: Dioecesis Truxillensis in Venetiola) is a diocese located in the city of Trujillo in the ecclesiastical province of Mérida in Venezuela.

On 4 June 1957 Pope Pius XII established the Diocese of Trujillo from the Metropolitan Archdiocese of Mérida.

The episcopal headquarters is in the city of Trujillo, where the Cathedral of Our Lady of Peace is located.

Its territory is divided into 79 parishes, a Rectory and a Vicariate.

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