The line was opened for service on 14 July 1856,[note 1] five trips a day, three in the morning and two in the afternoon, with a 28 minute traveling time.

To attract tourists, the York company built a building in Frascati, connected to Campitelli station, for concerts, receptions and a small theatre, but the initiative was unsuccessful and was abandoned.

The last section of the railway line and the new Frascati Station was inaugurated and opened for service on 2 February 1884, managed by Società per le Strade Ferrate Romane.

Frascati station was downgraded to a halt and the train service—unlike the Albano Laziale and Velletri lines, which were remotely controlled by the Ciampino signalling centre until May 2013 and then directly from Roma Termini—is operated as a shuttle.

Work began in 2011 on removing the concrete layer from track 1 and installing shelters on its platform in order to return it to service.