There are two features that distinguish it from both Peron's tree frog and Tyler's tree frog - the upper half of the iris is deep red, and it has no emerald green flecks on the dorsal surface.

Roth's tree frog breeds during the wet season, from November to March.

Eggs are laid in temporary pools of water, and the tadpoles take a maximum of 65 days to metamorphose.

The colour of Roth's tree frog is extremely variable, and can change from pale grey to dark brown within hours.

Typically, they are grey during the day whilst basking in the sun, and are brown at night.