[2] The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction.
In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has a C-terminal region rich in proline and serine residues.
binds to the core site, 5'-pygpyggt-3', of a number of enhancers and promoters.
The alpha-subunit binds DNA as a monomer, and appears to have a role in the development of normal hematopoiesis.
CBF is a nuclear protein expressed in numerous tissue types, except brain and heart; highest levels have been found to occur in thymus, bone marrow and peripheral blood.