[4] The fluorescence intensity increases linearly with the number of SYBR Gold molecules bound to DNA up to dye concentrations of ~ 2.5 μM, where quenching and inner filter effects become relevant.

The dissociation constant – a measure to describe the binding affinity of SYBR Gold to double-stranded DNA – is 0.27 ± 0.03 μM.

SYBR Gold can be readily removed from nucleic acids by ethanol precipitation, leaving pure templates available for subsequent manipulation or analysis.

Also, SYBR Gold can also be used for labelling of DNA within cells for flow cytometry and fluorescence microscopy.

Because SYBR Gold binds to DNA in an intercalative way with high affinity which makes it a possible carcinogen.