Saint Sebastian is a painting, once part of a triptych by the Italian Renaissance artist Antonello da Messina, completed in 1477–1479.

The visit of the Sicilian artist Antonello da Messina to Venice in 1475–1476 has traditionally been seen as turning point in the history of the city's painting, Giorgio Vasari crediting him with introducing oil painting to Venice.

However, art historians have generally accepted Antonello's authorship of the Saint Sebastian.

Original to Antonello is the smooth rendering of the body of Saint Sebastian (defying any geometrical breaking down) and the passion for the details of landscape, seen for example in the work of Umbrian painters such as Carlo Crivelli.

Famous details include the man reclining on the left, the typical Venetian-style chimneys, the columns and the monumental appearance of the buildings (probably inspired by some of Mantegna's works) and the debating pairs of men on the right side, forming an interesting mixing of late Gothic elements with Venetian, Flemish and advanced Renaissance ones.