The current church was built in 1869 as the Yeni Cami ("New Mosque") after the previous building was destroyed by the earthquake of 1856.

Various relics were brought to the church, among which the Holy Head of Titus and the icon of Virgin Mary Mesopanditissa.

[1] The church was then rebuilt in the style of a three-aisled basilica and was inaugurated by the archbishop of Crete, Fantino Dandolo, on January 3, 1446.

[1] The current building began to be constructed in 1869 according to a plan by Athanasios Moussis, who also designed, among others, the metropolitan church of Saint Menas.

[1] In 2013, the church of Saint Titus was made cathedral of the Archdiocese of Crete by Archbishop Irenaeus [el].