Their bodies were buried on the Via Aurelia and the church of Sante Rufina e Secunda was built in their honor in Rome.

[3] In the notes attached to the publication of Pope Paul VI's 1969 revision of the General Roman Calendar, it is stated that of these two saints, whose feast was inserted into the Roman Calendar in the 12th century on the occasion of the transfer of their relics to the Lateran Basilica, nothing is really known except their names and the fact that they were buried at the ninth milestone of the Via Cornelia.

[4] They are mentioned in the Bern manuscript of the "Martyrologium Hieronymianum," and are recorded also in seventh century "Itineraries" as on the Via Cornelia, where Pope Damasus I erected a Basilica over their grave.

[5] Sts Rufina and Secunda are sometimes depicted as two maidens floating in the Tiber River with weights attached to their necks.

In the 1620s, the Italian painters Il Morazzone, Giulio Cesare Procaccini, and Giovanni Battista Crespi collaborated on the "Martyrdom of Saints Rufina and Secunda," which was praised as "the painting by three hands" (Italian: "il quadro delle tre mani").