In 1538 the old monastery of "Preposturale of San Barnaba in Brova" by the Milan city wall was given to the congregation as their main seat, and thenceforth they were known the popular name of Barnabites.

It has a nave with barrel vault, finishing in a rectangular presbytery serving as the sanctuary.

The first mass was celebrated in 1568 by Archbishop of Milan Charles Borromeo, Cardinal Protector of the Barnabite order, who had himself donated the altar.

[4] There is an altar dedicated to Alexander Sauli, a Superior-General of the Barnabite order (1566–1569) and "Apostle of Corsica".

[4] The interior includes a notable selection of Milanese Mannerist artworks: the Stigmata of St. Francis by Giovan Paolo Lomazzo, a Pietà by Aurelio Luini and, flaking the high altar two large canvasses of Histories of St. Paul and Barnaba, Simone Peterzano's (1572–1573).