San Giustino is a comune (municipality) in the Province of Perugia in the Italian region Umbria, located about 50 kilometres (31 miles) northwest of Perugia on the Tiber River.

In ancient Roman times it was a town named Meliscianum.

Located in north of Umbria, next to the borders with Tuscany and Marche, the municipality borders with Borgo Pace (PU), Citerna, Città di Castello, Mercatello sul Metauro (PU) and Sansepolcro (AR).

It counts the hamlets (frazioni) of Celalba, Cospaia, Selci-Lama and Uselle-Renzetti.

San Giustino is twinned with: Media related to San Giustino at Wikimedia Commons This Umbria location article is a stub.