The Sanford Subdivision is a railroad line owned by CSX Transportation in Florida.
[1] Prior to 2011, the Sanford Subdivision continued south from DeLand along the A Line to Auburndale.
[2] In 2011, the Florida Department of Transportation bought the line between DeLand and Poinciana and operates the SunRail commuter rail service over that segment.
The remaining line south of Poinciana was annexed to CSX's Carters Subdivision after the sale.
The Sanford Subdivision today operates on tracks that were historically part of the Jacksonville, Tampa and Key West Railway.