Santa Ana National Wildlife Refuge is a 2,088-acre (8.45 km2) National Wildlife Refuge situated along the banks of the Rio Grande, south of Alamo in the Lower Rio Grande Valley, in Hidalgo County, South Texas.

Its unique location is at the meeting of different climates and habitats: subtropical wetlands, Chihuahuan Desert, Gulf Coast, and Great Plains.

The administration exempted the construction in the refuge, but built on the border of it, leading to possible interference due to noise.

Osprey, broad-winged hawk, northern harrier, and peregrine falcon are among the migratory birds of prey found in the refuge.

Hook-billed kite and gray hawk, seen occasionally in the refuge, attract birders from around the world.