The Santa Cruz Wildlife Refuge (Portuguese: Refúgio de Vida Silvestre de Santa Cruz) is a wildlife refuge in the state of Espírito Santo, Brazil.

The Santa Cruz Wildlife Refuge is offshore from the municipality of Aracruz, Espírito Santo.

The refuge covers a roughly rectangular area of the Atlantic Ocean extending southeast from the coast between Rio Preto in the south and Pontal do Piraqueaçu in the north.

[4] The refuge is classed as IUCN protected area category III (natural monument or feature).

[3] Objectives are to protect biological diversity and natural environments, including the mangroves and coastal vegetation important for species that use the area for feeding and breeding, and for stabilizing the coastline, to support compatible tourist, recreational and educational use of the seafront, and to contribute to recovery of biological resources and sustainability of fisheries and small-scale subsistence extraction by the coastal communities in the region around the unit.