The trail begins at the Santa Fe Depot, in the Railyard arts district, and ends at U.S. Route 285, near Lamy, New Mexico and the Lamy station.

The Santa Fe Rail Trail can be accessed via trailheads, connecting trails, and intersecting roadways in Santa Fe and Eldorado.

The New Mexico Rail Runner Express has stops on the trail at the Santa Fe Depot, South Capitol station, and Zia Road station.

The Gail Ryba Trail starts at Saint Michaels near the Hospital, joins the rail trail thru an underpass at Saint Francis Drive, and ends at West Zia Road.

The Spur Trail trailhead is located at the intersection of Richards Avenue and Avenida del Sur ( 35°35′56″N 106°00′05″W / 35.5988°N 106.0014°W / 35.5988; -106.0014 ).