The Saints compete in the Mid-Florida Conference within Region 8 of the National Junior College Athletic Association (NJCAA).

[7] Mallex Smith and Keon Broxton are Sante Fe alumni who have played in Major League Baseball (MLB).

[9] Santa Fe's women's softball team won the 2008 FSCAA Division I championship,[10] en route to being the runners-up in the NJCAA national tournament.

[12] In their history of intercollegiate competition, Santa Fe's athletic teams have won six NJCAA Region 8 state championships.

[13] The women's softball and men's baseball teams were national tournament runner-ups in their sport in 2008 and 2009, respectively.