Santa Maria Assunta is a Roman Catholic parish church located in Settignano, a frazione of Florence in the region of Tuscany, Italy.
The external facade is plain with only an early 16th-century terracotta with a Virgin and child with young St John.
The interior contains a fresco of the Resurrection by Maso da San Friano; in the chapel of the Holy Sacrament is a Last Supper (1615) by Andrea Commodi.
It also houses a baptismal font (1745), a 15th-century ciborium, and a wooden pulpit (1602) whose design is attributed to Bernardo Buontalenti and sculpture to Gherardo Silvani.
Adjacent to the church is the Oratory of the Holy Trinity, with frescoes of the Adoration of the Infant Jesus by a pupil of Desiderio da Settignano.