Route 227 (Hebrew: כביש 227, Kvish 227) is a 34-kilometre (21 mi) road in the eastern Negev desert of Israel.

"Scorpions' Ascent") is a steep, twisted section of Route 227, starting from the Tzafir stone structure (an archaeological site) in the south.

The Roman Empire built the ascent in the late 1st century CE from the Wadi Zin to the highlands of the northern Negev desert during their control of the Middle East.

[4] The Scorpion Path is mentioned in the Books of Numbers, Joshua, and Judges, in the Hebrew Bible, as the southern border of the territory given to the Tribe of Judah and of the Promised Land overall.

Route 227 passes through a series of nature reserves: Mishor Yamin, HaMakhtesh HaKatan, Makteshim-Ein Yahav and the Judean Desert.