It is located in the Murray Mallee subregion of the Murray-Darling Depression Bioregion, 150 km (93 mi) south of the city of Broken Hill.

Scotia comprises two former grazing properties, Tararra and Ennisvale, which were purchased by Earth Sanctuaries Limited in 1994, and eventually acquired by AWC in 2002.

[2] Scotia is characterised by a low-relief landscape of plains with parallel sand dunes and open calcareous swales.

[3] The climate is one of cool winters and hot summers in an arid region, with irregular rainfall averaging an annual 250 mm (9.8 in).

[6] Threatened bird species present on Scotia include malleefowl, regent and scarlet-chested parrots, striated grasswren and black-eared miner.