The Seymour line is a regional passenger rail service operated by V/Line in Victoria, Australia.

V/Line operates hourly passenger services from Seymour to Melbourne, using Sprinter and VLocity railcars.

Services generally stop all stations between Shepparton and Donnybrook in both directions.

Some services run express through Donnybrook, Heathcote Junction and Tallarook.

Platform 2 connects the Seymour and Shepparton services, which utilise the broad gauge (BG) track.