Sir William Twysden, 1st Baronet (1566–1628) was an English politician who sat in the House of Commons at various times between 1593 and 1628.
He was admitted to Gray's Inn on 28 October 1584 and succeeded his father in the estate at East Peckham in November 1603.
He was appointed a Gentleman Usher of the Privy Chamber to James I of England and knighted at Charterhouse on 11 May 1603.
Twysden was a great collector of manuscripts and knew Hebrew, Greek, and other languages.
His second son was Sir Thomas Twisden, 1st Baronet of a new creation, and a judge of the Court of King's Bench: he changed the spelling of the family name.