The series was produced by the Canadian animation studio Nerd Corps Entertainment, later part of DHX Media (now known as "WildBrain").

Deep inside the Earth exists the vast underground world of Slugterra, named after the tiny slugs who live there.

The Shane family are the traditional guardians of Slugterra and have kept its existence secret from the world as they train the slugs for use as living ammunition.

A threat appears in the form of Dr. Thaddeus Blakk, who wants to transform the Slugs into evil weapons he calls "ghouls."

The series, consisting of 20 four-minute-long shorts, explores the adventure of Eli and Trixie against Locke and Lode.