Sophie Amalie Moth, Countess of Samsøe (28 March 1654 – 17 January 1719) was the officially acknowledged royal mistress of King Christian V of Denmark.

The still-existing Danish noble family of Danneskiold-Samsøe is descended from her.

Sophie Amalie Moth was the first officially acknowledged royal mistress in Denmark.

Sophie Amalie was born on 28 March 1654 as the daughter of Poul Moth (1601–1670), doctor of the royal court, and Ida Dorothea Bureneus (1624–1684).

After Niels Juel's death in 1697, the king arranged for her to take over his mansion, today known as the Thott Palace after a later owner and housing the French Embassy in Copenhagen.