[2] Common side effects include pain at the area of injection, rash, nausea, fever, and trouble sleeping.

This ketone is then formed into a primary amine group through pyridoxal phosphate (PLP) and glutamine transamination (3a).

Once these two amines are present, the glucose ring is ready to be methylated through two S-adenosyl methionine molecules (5a).

While all this happens, an alternate pathway is occurring creating the sugar functional group from a similar starting product.

TDP glucose then has the hydroxyl removed from C6 through a hydratase enzyme (3b), which is then reduced through NADH at C4 creating a new product (4b).

This conversion to 6b is also accompanied by a double reduction at C4 and C3 through two more NADH molecules, giving the final product necessary to form the aminoglycoside.

With this, products 7a and 6b can come together, removing the TDP functional group and combining with the sugar molecule to form the aminoglycoside spectinomycin.

[citation needed] It is present in the genome or plastome of many plastids as the spc operon, which is usually two to 10 genes long.