[1] Set shortly after the events of the 2002 film,[2] Peter Parker, and his friends Mary Jane Watson, and Harry Osborn start attending Empire State University together.

Peter's superhero duties, and later his involvement with Indira Daimonji, interfere with his romance with Mary Jane, while Harry continuously blames Spider-Man for the death of his father Norman Osborn.

[6] Peter Parker was originally supposed to wear baggier clothes to hide his superhero musculature, but cost-effective difficulties with the CGI prevented folds from being put into his everyday attire.

[2] The character of Aunt May was not included in the series (except for a photograph in Peter's bedroom), because MTV executives feared that the appearance of any elderly people would deter their target youth audience from watching.

The entire series was licensed by Marvel and Sony to DigiKids/Sentimental Journeys, who re-edited the footage from many episodes into one feature, which is sold as a personalized DVD in which the purchaser's face is revealed under Spider-Man's mask.