Anny) is a Roman Catholic church in Vilnius' Old Town, on the right bank of the Vilnia river established circa 1495–1500.

St. Anne's is a prominent landmark in the Vilnius Old Town that enabled the district to be named as a UNESCO World Heritage Site,[1] and is one of the most interesting examples of Gothic architecture in Lithuania.

The first church at this site, constructed of wood, was built for Anna, Grand Duchess of Lithuania, the first wife of Vytautas the Great.

[4] Most recent reconstruction followed in 2009: the roofing was replaced, facade elements were reinforced and long-missing side spires were rebuilt.

On 13 June 2018 St. Anne's Church was dedicated by The Archbishop of Vilnius, Gintaras Grušas, to be used exclusively to celebrate the Tridentine Mass.

According to Lithuanian architect and art historian Vladas Drėma, patterns from the Columns of Gediminas are echoed in the church's façade.