In the 17th century, the prominent hymn-writer Paul Gerhardt was the minister of this church, and the composer Johann Crueger was musical director.

The prominent Lutheran theologian Provost Philipp Jacob Spener was the minister from 1691 to 1705.

Then the building, the oldest structure in Berlin proper, was given up to the government, to be used as a concert hall and ecclesiastical museum.

[2] The number of parishioners had shrunk due to the ever intensifying gentrification of the inner city, as residential premises became superseded by offices and shops.

During World War II, the Church of St. Nicholas had its roof and the tops of its towers destroyed as a result of Allied bombing.