Erected in the 1830s by United Empire Loyalists and Scottish immigrants on its current site, Princess St. at Clergy St., St. Andrew's Presbyterian was the first stone church in Kingston.

On June 9, 1831, St Andrew’s Church hosted a meeting of ministers and elders from four presbyteries: Quebec, Glengarry, Bathurst, and York.

[3] In 1912 the formal connection between the university and church was severed, but many of the traditions at Queen's still have elements of its Scottish Presbyterian heritage.

On April 8, 1888, a fire sparked by the church furnace caused the structure to burn to the ground, leaving only the thick stone walls standing.

One of the windows features the Arms of the Royal Military College of Canada and the line 'Dulce et decorum est pro patria mori'.

Home today to a church population of around 100–150, St. Andrews plays an active role in the Kingston community.

In the summer of 2009, St. Andrew's applied to the federal government for a grant to begin a tour guide program at the church.