U.S. Route 301 (US 301) is a part of the U.S. Highway System that runs from Sarasota, Florida, to Biddles Corner, Delaware.

US 301 replaced US 17–1 in the early 1930s and was extended from Petersburg north along its current course into Maryland when the Potomac River Bridge was completed in 1940.

US 301 enters Greensville County, Virginia from North Carolina as two-lane Skippers Road, which closely parallels CSX's North End Subdivision rail line, crosses Fontaine Creek, and passes through the hamlet of Skippers.

US 301 passes through downtown Emporia, crosses the Meherrin River, passing three Virginia Historical Markers in one block facing northbound traffic between Virginia Avenue and Valley Street, and has a grade crossing of Norfolk Southern Railway's Franklin District immediately to the south of its intersection with US 58 Business (Atlantic Street).

[1][2] South of Carson, US 301 enters Prince George County and its name changes to Crater Road.

The U.S. Highway crosses Warwick Swamp and has another interchange with I-95 in Kingwood, swapping to the west side of the Interstate.

US 301 enters the city of Petersburg and expands to a four-lane divided highway just south of Wagner Road.

[1][2] US 301 and US 1 pass through the city of Colonial Heights as Boulevard, a five-lane road with center turn lane.

The three highways cross Swift Creek into Chesterfield County, where SR 144 splits northwest as Harrowgate Road and the U.S.

Highways intersect SR 161 (Bells Road), which leads to the Port of Richmond on the James River.

The bridge crosses over CSX's Rivanna Subdivision before reaching a partial interchange with 2nd Street, allowing access to and from the south, at its northern end, west of the Tredegar Iron Works.

US 301 and US 1 follow Belvidere Street through the Oregon Hill neighborhood of Richmond, which lies to the east of Hollywood Cemetery, site of the James Monroe Tomb.

A short distance north of the city limits, the carriageways split, with the southbound lanes crossing to the west of I-95.

At Hanover Courthouse, the highway meets the eastern end of SR 54 (Patrick Henry Road).

After passing around the east side of the town of Bowling Green, the U.S. Highway receives the northern end of its business route, Broaddus Avenue.

Hill, US 301 passes the site of the Garrett farmhouse and barn where John Wilkes Booth was killed by federal troops 12 days after he had assassinated Abraham Lincoln.

US 301 becomes an undivided highway as it passes through the town of Port Royal as Main Street, then uses the James Madison Memorial Bridge to cross the Rappahannock River into King George County.

The U.S. Highway uses the Harry W. Nice Memorial Bridge to cross the Potomac River into Maryland.

[citation needed] Prior to 1933, the road from Petersburg south into North Carolina was State Route 24.

In March 1923, a delegation from Petersburg appeared before the State Highway Commission, requesting that this be added to the system.

The highway runs 2.96 miles (4.76 km) between junctions with US 301 in the southern and downtown sections of the city.

US 301 Alternate follows Sycamore Street between its southern junction with US 301 in the Walnut Hill neighborhood and Old Towne Petersburg.

The highway runs 1.50 miles (2.41 km) between junctions with US 301 on the south and east sides of Bowling Green.