Glotov's first recorded voyage was in 1746 aboard the vessel Ioann ("John"), belonging to the merchant Fyodor Kholodilov of Totma.
This ship sailed from the Kamchatka Peninsula, hunting for sea otters and seals, and wintered on Bering Island.
In the following spring, the Ioann went in search of the middle Aleutian Islands (the chain had fairly recently come to the awareness of the Russians), but were unsuccessful.
In 1758–1762, Glotov and Cossacks of the Nizhny Kamchatsky ostrog explored aboard the vessel Saint Julian.
[5] During this time (1759–1762), Glotov and his men discovered several islands of the Aleutian chain, including Unimak and Unalaska.
In October of 1768, Glotov was part of an expedition led by Krenitsyn and Levashov, which explored 200 kilometres (120 miles) of the northern coast of the Alaska Peninsula and discovered Port Moller Bay.