In 2010 he spent one year as the running backs coach with one season at Tennessee State before returning to Western Kentucky.
Holt spent 2013 and 2014 at the University of South Florida,[4] where he was serving as the Bulls' tight ends coach and special teams coordinator.
Holt was nominated for the Broyles Award at the end of the season after USF's special teams improved in almost every statistical category even with the loss of key performers.
Holt followed Scott Satterfield and was hired as the tight ends coach and special teams coordinator at Louisville in December 2018.
[9] Brent Pry announced on December 10, 2021, that Stu Holt would join his staff and serve as the special teams coordinator/running backs coach for the Virginia Tech Hokies.