The headwaters of the Suwannee River are in the Okefenokee Swamp in the town of Fargo, Georgia.

The confluences of these three rivers form the southern borderline of Hamilton County, Florida.

[17] The Suwannee River is a diverse and rich ecological space, hosting varied aquatic and wetland habitats.

It is home to a large number of temperate and subtropical species, including unique and endangered ones.

Fish & Wildlife Service, "The Lower Suwannee National Wildlife Refuge is unlike other refuges in that it was not established for the protection of a specific species, but in order to protect the high water quality of the historic Suwannee River.

"[20] The Suwannee River Wilderness Trail is "a connected web of Florida State Parks, preserves and wilderness areas" that stretches more than 170 miles (274 kilometers), from Stephen Foster Folk Culture Center State Park to the Gulf of Mexico.