The women's 400 metre freestyle competition at the 2014 South American Games took place on March 7 at the Estadio Nacional.

[1][2] The last champion was Joanna Maranhão of Brazil.

[3] This race consisted of eight lengths of the pool, with all eight being in the freestyle stroke.

[4] Prior to this competition, the existing world and Pan Pacific records were as follows: All times are in minutes and seconds.

[1] The final was held on March 7, at 19:10.