TMP-HTag

Trimethoprim-Halotag (TMP-HTag) is a small molecule chemical linker developed for the rapid and reversible control of protein localization in living cells (Ballister).

[1][2] The other half of the linker is a Halotag, a self labelling bacterial globular protein ligand that can bind covalently and irreversibly to the chloroalkane group of a Haloenzyme.

[2][3] Positioned between the TMP group and HaloTag is a flexible linker that can be modified to optimize protein linking efficiency.

[2][3] The modular structure of TMP-HaloTag makes it an ideal heterobifunctional tool for use in chemically induced dimerization (CID).

[1][2] Additionally, TMP- HTag can be modified to include photo-cleavable groups that allow for the control of CID using light.

Chemical structure of the TMP-HTag linker ^{[

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