TSI slant

All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.

Examples of sulfide-producing bacteria include Salmonella, Proteus, Citrobacter and Edwardsiella species.

Whereas if it ferments both lactose and glucose, it results in a yellow/yellow tube and remains that way due to the large amount of acid produced in the reaction.

Salmonella enterica serovar Typhi may result in blackening of the medium at the interface of bottom and the slant.

Under anaerobic conditions (as occur toward the bottom of the tube) some bacteria use thiosulfate as an electron acceptor and reduce it to hydrogen gas.