Her other notable films include Diary of the Dead (2007), Eastern Promises (2007), The Vow (2012), Picture Day (2012), Cas and Dylan (2013), Woman in Gold (2015), Stronger (2017), and Destroyer (2018).

[6][9] For elementary school, Maslany was in French immersion and was taught in German by her mother before learning English.

[14][15] While attending high school, she found paying acting jobs that allowed her to travel all over Canada with her parents' approval.

"[10] After completing high school, she took a gap year before entering the University of Regina, studying German, Ancient Greek, Philosophy, Psychology, and Film.

[20] In 2007, Maslany appeared in The Messengers and had a recurring role for three seasons on the CBC series Heartland.

Also in 2010, she appeared as the protagonist Mary, the mother of Jesus, in the British four-part television series The Nativity.

In 2012, Maslany appeared as lead character Claire in Picture Day, for which she won a Phillip Borsos Award for Best Performance at the 2012 Whistler Film Festival.

[30] Maslany guest starred on the season 39 finale of Saturday Night Live and played the character Bridget in the episode's second digital short entitled "Hugs".

[31] Maslany co-starred in the 2015 film Woman in Gold as a younger version of Maria Altmann, Helen Mirren's character.

[36] In the 2017 film Stronger, Maslany starred as Erin Hurley, the love interest of Jeff Bauman, a victim of the Boston Marathon bombing.

Maslany starred as Sister Alice in the HBO period drama miniseries Perry Mason, which premiered in June 2020.

[40] She was set to star in AMC's six-episode series Invitation to a Bonfire, a psychological thriller set in the 1930s at an all-girls boarding school based on a 2018 novel by Adrienne Celt,[41] but production on the series was cancelled by the network for content tax write-downs in January 2023, with apparently four episodes shot.