It begins at an intersection with Round Mountain Road south of Del Rio and ends at the North Carolina state line east of Unicoi, where it becomes NC 226.

SR 107 goes northwest through narrow valley and mountains to enter Del Rio, where it crosses a bridge over the French Broad River and becomes concurrent with US 25/US 70/SR 9.

They then go east to leave Del Rio and parallel the river to an intersection where SR 107 splits off and goes north.

The highway then passes through a rugged and remote part of the Cherokee National Forest before crossing into Greene County.

They then come to an intersection where SR 107 splits off and goes southeast, becoming a primary highway and leaving Tusculum.

The highway then becomes very curvy coming to the North Carolina state line, where SR 107 ends and continues east as NC 226.

[4] Flooding from Hurricane Helene on September 27, 2024 caused the Nolichucky River to over flow its banks which led to the Elbert Kinser Bridge collapsing in Greene County.