The length of the route is an estimated total of 18.0 miles (29.0 km) through Pickett and northwestern Fentress Counties.

[3] The western terminus of SR 325 is located on the eastern shores of Dale Hollow Lake, at the Star Point Resort in western Pickett County.

[3] SR 325 west of Byrdstown was originally a part of SR 53 that served as a direct route from Byrdstown to Celina, in Clay County until sometime around 1943, when the U.S. Army Corps of Engineers impounded the Obey River to create Dale Hollow Lake.

The lake's formation disconnected the roadway between the two cities, and parts of SR 53 in that area, along with most of the town of Willow Grove, is now under water.

After the dam was built in Clay County, SR 53 was rerouted to the Kentucky state line to become a then-new alignment of KY 61, which also followed a path directly into the lake.