It connects the community of Culleoka with the cities of Lewisburg and Columbia, along with I-65.

The highway winds its way east through more hilly terrain for a few miles to cross into Marshall County.

[3] SR 373 winds its way through rural hilly countryside for several more miles as Mooresville Highway, where it has an interchange with I-65 (Exit 32) and widens to a four-lane undivided highway, before entering Lewisburg at an intersection with SR 417 (W Ellington Parkway).

The highway becomes W Commerce Street and passes through neighborhoods for several miles before entering downtown.

SR 373 then comes to an end at the courthouse square at an intersection with US 31A Business/US 431 Business/SR 11/SR 50 (2nd Avenue/E Commerce Street).