After waiting 30 seconds and ensuring that no adverse reactions have occurred, the remaining 8 mg of edrophonium is also injected.

The patient is then asked to perform repetitive tasks, such as lifting an object or opening and closing the eyelids.

Under the influence of edrophonium, patients with myasthenia gravis are able to complete these tasks more easily and with more strength than before the injection was administered.

A thorough history taking and physical assessment is done before the administration of test, to rule out hypersensitivity, pregnancy, lactation, and intestinal obstruction.

Following the test, the patient is observed for cholinergic responses such as increased salivation, lacrimation, nausea, and vomiting.