tert-Butyl bromide (also referred to as 2-bromo-2-methylpropane) is an organic compound with the formula Me3CBr (Me = methyl).

The molecule features a tert-butyl group attached to a bromide substituent.

This organobromine compound is used as a standard reagent in synthetic organic chemistry.

Illustrative is the tert-butylation of cyclopentadiene to give di-tert-butylcyclopentadiene:[3] tert-Butyl bromide used to study the massive deadenylation of adenine based-nucleosides induced by halogenated alkanes (alkyl halides) under physiological conditions.

2-Bromo-2-methylpropane causes the massive deguanylation of guanine based-nucleosides and massive deadenylation of adenine based-nucleosides.