[1] It is almost exclusively located in the probable 5' untranslated regions of protein-coding genes, and most of these genes are known to encode either folate transporters or enzymes involved in folate metabolism.

THF riboswitches are found in a variety of Bacillota, specifically the orders Clostridiales and Lactobacillales, and more rarely in other lineages of bacteria.

The THF riboswitch was one of many conserved RNA structures found in a project based on comparative genomics.

[2] The 3-d structure of the tetrahydrofolate riboswitch has been solved by separate groups using X-ray crystallography.

[3][4] These structures were deposited into the Protein Data Bank under accessions 3SD1 and 3SUX, with other entries containing variants.