SH 107 begins at an intersection with Interstate 2/US Route 83 in Mission and travels north through the center of town.

It reaches an intersection with the old routing of US 83 and Texas State Highway 495 in Mission before exiting to the north.

It continues east through rural farmlands, passing through the towns of Elsa, Edcouch, and La Villa.

The then turn back to the east, reaching the intersection of Business Route 77 and the eastern terminus at I-69E/US 77 in Combes.

[9] The route has changed little since then, with the stretch from Mission to Hidalgo being transferred to FM 1016 on November 23, 1948.